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Literature summary extracted from

  • Pandey, R.; Parajuli, P.; Chu, L.; Darsandhari, S.; Sohng, J.
    Biosynthesis of amino deoxy-sugar-conjugated flavonol glycosides by engineered Escherichia coli (2015), Biochem. Eng. J., 101, 191-199.
No PubMed abstract available

Application

EC Number Application Comment Organism
2.6.1.33 synthesis engineering of Escherichia coli to divert the flow of carbon flux from glucose-1-phosphate to thymidine diphosphate 4-keto 4,6-dideoxy-D-glucose (dTKDG), an intermediate of various dTDP-sugars. Glucose phosphate isomerase, glucose-6-phosphate dehydrogenase and uridylyltransferase genes are deleted while dTDP-D-glucose synthase and dTDP-Dglucose 4,6-dehydratase are overexpressed to produce a pool of dTKDG in the cell cytosol. The flow of dTKDG is further diverted to dTDP-D-viosamine, dTDP 4-amino 4,6-dideoxy-D-galactose, and dTDP 3-amino 3,6-dideoxy-D-galactose sugars using sugar aminotransferases gerB, wecE, and fdtB, respectively, from different sources. These sugar moieties are transferred to the 3-hydroxyl position of quercetin and kaempferol with the help of Arabidopsis thaliana glycosyltransferase ArGT3 Escherichia coli
2.6.1.90 synthesis engineering of Escherichia coli to divert the flow of carbon flux from glucose-1-phosphate to thymidine diphosphate 4-oxo 6-deoxy-D-glucose (dTKDG), an intermediate of various dTDP-sugars. Glucose phosphate isomerase, glucose-6-phosphate dehydrogenase and uridylyltransferase genes are deleted while dTDP-D-glucose synthase and dTDP-D-glucose 4,6-dehydratase are overexpressed to produce a pool of dTKDG in the cell cytosol. The flow of dTKDG is further diverted to dTDP-D-viosamine, dTDP 4-amino 4,6-dideoxy-D-galactose, and dTDP 3-amino 3,6-dideoxy-D-galactose sugars using sugar aminotransferases gerB, wecE, and fdtB, respectively, from different sources. These sugar moieties are transferred to the 3-hydroxyl position of quercetin and kaempferol with the help of Arabidopsis thaliana glycosyltransferase ArGT3 Aneurinibacillus thermoaerophilus

Organism

EC Number Organism UniProt Comment Textmining
2.6.1.33 Escherichia coli P27833
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-
2.6.1.90 Aneurinibacillus thermoaerophilus Q6T1W6
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-

Synonyms

EC Number Synonyms Comment Organism
2.6.1.33 WecE
-
 Escherichia coli
2.6.1.90 FdtB
-
 Aneurinibacillus thermoaerophilus

Cofactor

EC Number Cofactor Comment Organism Structure
2.6.1.90 additional information enzyme utilizes an oxidized quinone as co-factor Aneurinibacillus thermoaerophilus